Isolation and Identification of Eschericia Coli in Raw and Pasteurized Milk Sold Within Jalingo Metropolis and Environs

  • Tirmidhi, A.B.
  • Aboluja, A.B.
  • Umar, Y. J.
  • Hureira, A. T.
  • Haulatu, B. A.
Keywords: Raw Milk, E. coli, Isolation, Identification, Jalingo


A study was carried out to determine the presence of Escherichia coli in raw and pasteurized milk from commercial vendors sold within Jalingo metropolis and nearby villages. A total of 50 samples were collected and processed bacteriologically, cultured in Eosine Methylene Blue agar medium and the isolates were tested for E.coli. using biochemical tests. The study revealed that 40% of the collected samples were contaminated with E. coli. Besides, all E. coli isolates exhibited bright pink colour on Eosin Methylene Blue agar plate and gram-negative, pink-colored, small rod-shaped organisms arranged on Gram’s staining. In this study, there was no significant (p< 0.05) between the result obtained from Jalingo and Ardo kola with the isolates of E. coli. In conclusion, the presence and consumption of raw milk may constitute a public health hazard and reduced milk quality due to E. coli. Thus, health professionals should create awareness about milk handling practice, storage and milking process to Dairy farmer and milk collectors.


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How to Cite
Tirmidhi, A.B., Aboluja, A.B., Umar, Y. J., Hureira, A. T., & Haulatu, B. A. (2023). Isolation and Identification of Eschericia Coli in Raw and Pasteurized Milk Sold Within Jalingo Metropolis and Environs. Nigerian Journal of Animal Science and Technology (NJAST), 6(3), 87 - 93. Retrieved from