Isolation and Identification of Eschericia Coli in Raw and Pasteurized Milk Sold Within Jalingo Metropolis and Environs
Keywords:
Raw Milk, E. coli, Isolation, Identification, Jalingo
Abstract
A study was carried out to determine the presence of Escherichia coli in raw and pasteurized milk from commercial vendors sold within Jalingo metropolis and nearby villages. A total of 50 samples were collected and processed bacteriologically, cultured in Eosine Methylene Blue agar medium and the isolates were tested for E.coli. using biochemical tests. The study revealed that 40% of the collected samples were contaminated with E. coli. Besides, all E. coli isolates exhibited bright pink colour on Eosin Methylene Blue agar plate and gram-negative, pink-colored, small rod-shaped organisms arranged on Gram’s staining. In this study, there was no significant (p< 0.05) between the result obtained from Jalingo and Ardo kola with the isolates of E. coli. In conclusion, the presence and consumption of raw milk may constitute a public health hazard and reduced milk quality due to E. coli. Thus, health professionals should create awareness about milk handling practice, storage and milking process to Dairy farmer and milk collectors.
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References
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Abera, L. (2008). Study on Milk Production and Traditional Dairy Handling Practices in East Shoa Zone, Ethiopia [M.S. thesis], Faculty of Veterinary Medicine, Addis Ababa University. Pp:55-91
Acha, P.N. and Szyfress, B. (2001). Zoonoses and communicable diseases common to man and animals, Bacteriosis and mycoses. 3rd ed. Washington, D.C: Pan American Sanitary Bureau, Pp: 12-30.
Asmelash, T. (2015). Isolation, Identification, Antimicrobial Profile and Molecular Characterization of Enterohaemorrhagic E. coli O157: H7 Isolated from Ruminants Slaughtered at Debre Zeit Elfora Export Abattoir and Addis Ababa Abattoirs Enterprise. [M.S.c thesis], Faculty of Veterinary Medicine, Addis Ababa University.
Battisti, A., Lovari, S., Franco, A., Diegidio, A., Tozzoli, R., Caprioli, A.and Morabito, S. (2006). Prevalence of Escherichia coli O157 in Lambs at Slaughter in Rome, Central Italy. Epidemiology of Infection, 134: 415–419.
Bedasa, S., Daniel, S., Ashebr, A.and Tesfanesh, M. (2018): Occurrence and Antimicrobial Susceptibility Profile of Escherichia Coli O157: H7 from Food of Animal Origin in Bishoftu Town, Central Ethiopia. International Journal of Food Contamination 5:2.
Biruke, D. and Shimeles, A. (2015). Isolation and Identification of Major Bacterial Pathogen from Clinical Mastitis Cow Raw Milk in Addis Ababa, Ethiopia. Academic Journal of Animal Diseases 4(1): 44-51.
Carbas, B., Cardoso, L., Coelho, A. (2012). Investigation on the knowledge associated with foodborne diseases in consumers of northeastern Portugal. Food Control. 30(1): 54-7.
CLSI., (2008). Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria: Approved guideline. CLSI Guideline M45-A, Clinical and Laboratory Standards Institute, Wayne, PA., USA.
Doyle, M., Archer, J., Kaspar, C. and Weiss, R. (2006). Human illness caused by E. coli O157: H7 from food and non-food sources. FRI briefings.
Fairbrother, J., Batisson, I., Girard, F., Mellata, M. and Pérès, S. (2002). Original text on E. coli. Animal Health and Production Compendium, CD-ROM CAB International, Pp:141-147
FAO and WHO, (2004): Code of hygienic practice for milk and milk products, AC/RCP 57, Codex Alimentarius, Rome, Italy
Gwida, M.M. and EL-Gohary, FA. (2013). Zoonotic Bacterial Pathogens Isolated from Raw Milk with Special Reference to Escherichia coli and Staphylococcus aureus in Dakahlia Governorate, Egypt. Open Access Scientific Reports, 2: 705
Ji-Yeon, K., So-Hyun, K., Nam-Hoon, K., Won-Ki, B., Ji-Youn, L, Hye-Cheong, K., JunMan, K., Kyoung, N., Woo-Kyung, J., Kun-Taek, P. and Yong-Ho, P.(2005). Isolation and identification of Escherichia coli O157: H7 using different detection methods and molecular determination by multiplex PCR and RAPD. J vet sci, 6(1): 7–19.
Karmali, M.A., (1989). Infection by verocytotoxin-producing Escherichia coli. Clinical microbiology reviews, 2(1):15-38.
Leedom, J. (2006). Milk of non-human origin and infectious diseases in humans. Clinically Infected Disease, 43:610-615
LeJeune JT, Rajala-Schultz PJ (2009). Food safety: Unpasteurized milk: a continued public health threat. Clinically Infected Disease 48: 93-100.
Mora, A., León, S., Blanco, M., Blanco, J., López, C., Dahbi, G., Echeita, A., González, E. and Blanco, J. (2007). Phage types, virulence genes and PFGE profiles of shiga toxin-producing E.coli O157: H7 isolated from raw beef, soft cheese and vegetables in Lima (Peru). International Journal Food Microbiology, 114(2): 204-253.
Muragkar, H.V., Rahman, H., Ashok, K., Bhattacharyya, D. (2004). Isolation, phage typing and antibiogram of Salmonella from man and animals in north-eastern India. Indian Journal of Medical Research 122:237 – 242.
Nataro, J.P. and Kaper, J.B., (1998). Diarrheagenic Escherichia coli. Clinical microbiology reviews, 11(1):142-201.
Nigatu, D., Birhanu, S., Shimelis, M., Yimer, M., and Dinaol, B. (2017). Prevalence and Antimicrobial Susceptibility Pattern of E. coli O157: H7 Isolated from Traditionally Marketed Raw Cow Milk in and around Asosa Town, Western Ethiopia. Hindawi Veterinary Medicine International Volume, Article ID 7581531, 7
Oliver, S.P., Jayarao, B.M. and Almeida, R.A., (2005): Foodborne pathogens in milk and the dairy farm environment: food safety and public health implications. Foodbourne Pathogens & Disease, 2(2):115-129.
Perelle, S., Dilasser, F., Grout, J., and Fach, P. (2007). Screening food raw materials for the presence of the world’s most frequent clinical cases of Shiga toxin encoding Escherichia coli I O26, O103, O111, O145 and O157. International Journal Food Microbiology, 11:284-288.
Reta, M.A., Bereda, T.W. and Alemu, A.N., (2016). Bacterial contaminations of raw cow’s milk consumed at Jigjiga City of Somali Regional State, Eastern Ethiopia. International Journal of Food Contamination, 3(1): 4.
Rey, J., Blanco, J.E., Blanco, M., Mora, A., Dahbi, G., Alonso, J.M., Hermoso, M., Hermoso, J., Alonso, M.P., Usera, M.A. and González, E.A., (2003). Serotypes, phage types and virulence genes of Shiga-producing Escherichia coli isolated from sheep in Spain. Veterinary microbiology, 94(1): 47-56.
Rundasa, M., Mesfin M. and Haben, F. (2019). Isolation and Identification of Escherichia coli from Dairy Cow Raw Milk in Bishoftu Town, Central Ethiopia. Archives of Veterinary and Animal Sciences, 1(1): 1-7
Saba C.K.S., Eric Y and Adzitey F. (2015). Prevalence of Escherichia coli and Shiga Toxin-Producing Escherichia coli in Cattle Faeces and Raw Cow Milk Sold in the Tamale Metropolis, GHANA. Journal Dairy Veterinary Animal Research, 2(5): 37-92
Taraba State Diary (2021). Taraba State Diary.
Tchaptchet, S. and Hansen, J., (2011). The Yin and Yang of host-commensal mutualism. Gut Microbes, 2(6): 347-352.
Virpari, P., Nayak, J., Thaker, H.and Brahmbhatt, M. (2013). Isolation of pathogenic Escherichia coli from stool samples of diarrhoeal patients with history of raw milk consumption, Veterinary World, 6(9): 659-663
Woynshet, H. (2014). Escherichia Coli O157:H7: Prevalence and Sources of Contamination of Cattle Meat at Municipal Abattoir and Butcheries as well as its Public Health Importance in Addis Ababa, Ethiopia [M.S. thesis], Faculty of Veterinary Medicine, Addis Ababa University, Pp. 25-56.
Yilma, Z. and Faye, B., (2006). Handling and microbial load of cow’s milk and Irgo-fermented milk collected from different shops and producers in central highlands of Ethiopia. Ethiopian Journal of Animal Production, 6(2): 67-82.
Published
2023-11-13
How to Cite
Tirmidhi, A.B., Aboluja, A.B., Umar, Y. J., Hureira, A. T., & Haulatu, B. A. (2023). Isolation and Identification of Eschericia Coli in Raw and Pasteurized Milk Sold Within Jalingo Metropolis and Environs. Nigerian Journal of Animal Science and Technology (NJAST), 6(3), 87 - 93. Retrieved from http://njast.com.ng/index.php/home/article/view/281
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